Journal: Advanced Techniques in Diagnostic Microbiology

Article Title: Multiplex PCR for Detection and Identification of Microbial Pathogens

doi: 10.1007/978-3-319-95111-9_19

Figure Lengend Snippet: Multiplex nucleic acid tests: FDA-cleared and/or CE-marked

Article Snippet: Similarly, in a pre-/post-intervention study of pediatric patients admitted through the ED, Rodgers et al. [ ] compared several outcome measures when the FilmArray RP replaced the use of three clinician-ordered traditional PCR tests (Prodesse assays for, FluA/B/RSV, PIV 1,2,3, and hMPV).

Techniques: Multiplex Assay, Luminex, Labeling, Hybridization, Microarray

Journal: Advanced Techniques in Diagnostic Microbiology

Article Title: Multiplex PCR for Detection and Identification of Microbial Pathogens

doi: 10.1007/978-3-319-95111-9_19

Figure Lengend Snippet: The FilmArray Pouch: ( a ) Picture of a pouch hydrated with blue dye (through the left-side sample port) and red dye (through the right-side hydration port). In the film portion of the pouch, white zirconium beads (#1) are located in front of a yellow rectangle of tape which protects the pouch during bead beating. Black silica magnetic beads (#2) bind nucleic acid from the sample. A white pill of PCR primers (#3) is used in the first stage PCR. The black array (#4) contains 102 wells of 1 microliter each for 96 PCR reactions plus control empty wells. ( b ) Schematic of the pouch indicating the different functional sections of the pouch

Article Snippet: Similarly, in a pre-/post-intervention study of pediatric patients admitted through the ED, Rodgers et al. [ ] compared several outcome measures when the FilmArray RP replaced the use of three clinician-ordered traditional PCR tests (Prodesse assays for, FluA/B/RSV, PIV 1,2,3, and hMPV).

Techniques: Magnetic Beads, Functional Assay

Journal: Advanced Techniques in Diagnostic Microbiology

Article Title: Multiplex PCR for Detection and Identification of Microbial Pathogens

doi: 10.1007/978-3-319-95111-9_19

Figure Lengend Snippet: Schematic of nested multiplex PCR as performed in the FilmArray pouch. Reverse transcription and the first-stage multiplex PCR occur in a blister of the pouch (shown in pink). Primers spotted into wells of the array (shown in blue) allow singleplex nested PCRs to occur in each well

Article Snippet: Similarly, in a pre-/post-intervention study of pediatric patients admitted through the ED, Rodgers et al. [ ] compared several outcome measures when the FilmArray RP replaced the use of three clinician-ordered traditional PCR tests (Prodesse assays for, FluA/B/RSV, PIV 1,2,3, and hMPV).

Techniques: Multiplex Assay

Journal: Advanced Techniques in Diagnostic Microbiology

Article Title: Multiplex PCR for Detection and Identification of Microbial Pathogens

doi: 10.1007/978-3-319-95111-9_19

Figure Lengend Snippet: Percent detection of each FilmArray RP pathogen in the FilmArray Trend RP dataset displayed as a stacked area graph for the July 2013 to July 2017 time period

Article Snippet: Similarly, in a pre-/post-intervention study of pediatric patients admitted through the ED, Rodgers et al. [ ] compared several outcome measures when the FilmArray RP replaced the use of three clinician-ordered traditional PCR tests (Prodesse assays for, FluA/B/RSV, PIV 1,2,3, and hMPV).

Techniques:

Journal: Diagnostics

Article Title: Comparative Evaluation of Allplex Respiratory Panels 1, 2, 3, and BioFire FilmArray Respiratory Panel for the Detection of Respiratory Infections

doi: 10.3390/diagnostics12010009

Figure Lengend Snippet: Comparison of the Allplex RP 1, 2, 3, and the BioFire FilmArray RP assays for the detection of respiratory viruses in nasopharyngeal swab (NPS) specimens (n = 181). ND: No virus detected.

Article Snippet: The difference in quantity (Ct value) of detected analytes was not compared because the BioFire FilmArray RP assay provided results as detected or not detected without Ct values.

Techniques:

Journal: Diagnostics

Article Title: Comparative Evaluation of Allplex Respiratory Panels 1, 2, 3, and BioFire FilmArray Respiratory Panel for the Detection of Respiratory Infections

doi: 10.3390/diagnostics12010009

Figure Lengend Snippet: Comparison of Allplex RP 1, 2, 3 and BioFire FilmArray RP assays for the detection of respiratory viruses in NPS specimens.

Article Snippet: The difference in quantity (Ct value) of detected analytes was not compared because the BioFire FilmArray RP assay provided results as detected or not detected without Ct values.

Techniques:

Journal: Diagnostics

Article Title: Comparative Evaluation of Allplex Respiratory Panels 1, 2, 3, and BioFire FilmArray Respiratory Panel for the Detection of Respiratory Infections

doi: 10.3390/diagnostics12010009

Figure Lengend Snippet: Cycle threshold (Ct) of M. pneumoniae -positive NPS specimens (n = 21) obtained from the Seegene PneumoBacter ACE Detection Panel. A complete concordance between the BioFire FilmArray RP and PneumoBacter ACE detection panel for the detection of M. pneumoniae was observed.

Article Snippet: The difference in quantity (Ct value) of detected analytes was not compared because the BioFire FilmArray RP assay provided results as detected or not detected without Ct values.

Techniques:

Journal: Diagnostics

Article Title: Comparative Evaluation of Allplex Respiratory Panels 1, 2, 3, and BioFire FilmArray Respiratory Panel for the Detection of Respiratory Infections

doi: 10.3390/diagnostics12010009

Figure Lengend Snippet: Cycle threshold (Ct) values of viruses detected in NPS specimens by the Allplex RP 1, 2, 3 assays. The Allplex RP assays generate Ct values of respiratory pathogens, which were accepted as correct and utilized to compare the performance against the BioFire FilmArray RP. The BioFire FilmArray RP assay does not differentiate between HEV and HRV as well as RSV-A, and RSV-B, and reports them as a single analyte HEV/HRV and RSV-A/RSV-B, respectively. The NPS specimens were ordered by ascending Ct values. Blue symbols denote NPS specimens positive on Allplex RP but negative on the BioFire FilmArray RP.

Article Snippet: The difference in quantity (Ct value) of detected analytes was not compared because the BioFire FilmArray RP assay provided results as detected or not detected without Ct values.

Techniques: RP Assay

Journal: Journal of Infection and Chemotherapy

Article Title: Evaluation of FilmArray respiratory panel multiplex polymerase chain reaction assay for detection of pathogens in adult outpatients with acute respiratory tract infection

doi: 10.1016/j.jiac.2018.05.006

Figure Lengend Snippet: Results of FilmArray.

Article Snippet: In the previous study on FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVP v1, and Luminex Fast Multiplex Assays, the consensus positive rate of pathogens in patients with URTI was 80.5% .

Techniques: Virus

Journal: Journal of Infection and Chemotherapy

Article Title: Evaluation of FilmArray respiratory panel multiplex polymerase chain reaction assay for detection of pathogens in adult outpatients with acute respiratory tract infection

doi: 10.1016/j.jiac.2018.05.006

Figure Lengend Snippet: Time-Series data of FilmArray RP . A prospective observational study was conducted between January 15 and April 5, 2016. During the influenza epidemics, respiratory syncytial virus, human rhinovirus , and coronavirus were detected.

Article Snippet: In the previous study on FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVP v1, and Luminex Fast Multiplex Assays, the consensus positive rate of pathogens in patients with URTI was 80.5% .

Techniques: Virus

Journal: Journal of Infection and Chemotherapy

Article Title: Evaluation of FilmArray respiratory panel multiplex polymerase chain reaction assay for detection of pathogens in adult outpatients with acute respiratory tract infection

doi: 10.1016/j.jiac.2018.05.006

Figure Lengend Snippet: FilmArray RP results in each clinical diagnosis . The clinical diagnosis was determined by the physicians without the FilmArray RP results. (A) In patients with acute upper respiratory tract infection, the most common pathogen was influenza virus . In two patients, two pathogens were detected: influenza virus and respiratory syncytial virus and influenza virus and human Rhinovirus. (B) In patients with acute bronchitis, influenza virus (n = 1), respiratory syncytial virus (n = 2), and human metapneumovirus (n = 2) were detected in the FilmArray RP. (C) In patients with pneumonia, 73% were negative in the FilmArray RP.

Article Snippet: In the previous study on FilmArray RP, Genmark eSensor RVP, Luminex xTAG RVP v1, and Luminex Fast Multiplex Assays, the consensus positive rate of pathogens in patients with URTI was 80.5% .

Techniques: Biomarker Discovery, Infection, Virus

Journal: Revue Francophone Des Laboratoires

Article Title: Les infections à coronavirus humains

doi: 10.1016/S1773-035X(16)30369-0

Figure Lengend Snippet: Trousses commerciales permettant la détection par PCR de pathogènes respiratoires, incluant les HCoV classiques et nouveaux.

Article Snippet: FilmArray RP , BioMerieux, France.

Techniques: Luminex

Journal: Frontiers in Microbiology

Article Title: Laboratory Diagnosis of Respiratory Tract Infections in Children – the State of the Art

doi: 10.3389/fmicb.2018.02478

Figure Lengend Snippet: Commercially available nucleic acid amplification assays for respiratory pathogens a .

Article Snippet: FDA-cleared commercial POC assays are limited to the detection of influenza A/B and RSV viruses, with the exception of the FilmArray ® RP EZ (BioFire) which detects 14 targets ( ).

Techniques: Amplification, Luminex, Quantitative RT-PCR, Multiplex Assay, Microarray, DNA Hybridization, Reverse Transcription Polymerase Chain Reaction, Hybridization, Real-time Polymerase Chain Reaction

Journal: Diagnostics

Article Title: Comparative Evaluation of Allplex Respiratory Panels 1, 2, 3, and BioFire FilmArray Respiratory Panel for the Detection of Respiratory Infections

doi: 10.3390/diagnostics12010009

Figure Lengend Snippet: Comparison of the Allplex RP 1, 2, 3, and the BioFire FilmArray RP assays for the detection of respiratory viruses in nasopharyngeal swab (NPS) specimens (n = 181). ND: No virus detected.

Article Snippet: The NPS specimens positive for M. pneumoniae on the BioFire FilmArray RP were further confirmed by a separate Seegene PneumoBacter ACE detection panel, which also offers Ct values for the detected analytes.

Techniques:

Journal: Diagnostics

Article Title: Comparative Evaluation of Allplex Respiratory Panels 1, 2, 3, and BioFire FilmArray Respiratory Panel for the Detection of Respiratory Infections

doi: 10.3390/diagnostics12010009

Figure Lengend Snippet: Comparison of Allplex RP 1, 2, 3 and BioFire FilmArray RP assays for the detection of respiratory viruses in NPS specimens.

Article Snippet: The NPS specimens positive for M. pneumoniae on the BioFire FilmArray RP were further confirmed by a separate Seegene PneumoBacter ACE detection panel, which also offers Ct values for the detected analytes.

Techniques:

Journal: Diagnostics

Article Title: Comparative Evaluation of Allplex Respiratory Panels 1, 2, 3, and BioFire FilmArray Respiratory Panel for the Detection of Respiratory Infections

doi: 10.3390/diagnostics12010009

Figure Lengend Snippet: Cycle threshold (Ct) of M. pneumoniae -positive NPS specimens (n = 21) obtained from the Seegene PneumoBacter ACE Detection Panel. A complete concordance between the BioFire FilmArray RP and PneumoBacter ACE detection panel for the detection of M. pneumoniae was observed.

Article Snippet: The NPS specimens positive for M. pneumoniae on the BioFire FilmArray RP were further confirmed by a separate Seegene PneumoBacter ACE detection panel, which also offers Ct values for the detected analytes.

Techniques:

Journal: Diagnostics

Article Title: Comparative Evaluation of Allplex Respiratory Panels 1, 2, 3, and BioFire FilmArray Respiratory Panel for the Detection of Respiratory Infections

doi: 10.3390/diagnostics12010009

Figure Lengend Snippet: Cycle threshold (Ct) values of viruses detected in NPS specimens by the Allplex RP 1, 2, 3 assays. The Allplex RP assays generate Ct values of respiratory pathogens, which were accepted as correct and utilized to compare the performance against the BioFire FilmArray RP. The BioFire FilmArray RP assay does not differentiate between HEV and HRV as well as RSV-A, and RSV-B, and reports them as a single analyte HEV/HRV and RSV-A/RSV-B, respectively. The NPS specimens were ordered by ascending Ct values. Blue symbols denote NPS specimens positive on Allplex RP but negative on the BioFire FilmArray RP.

Article Snippet: The NPS specimens positive for M. pneumoniae on the BioFire FilmArray RP were further confirmed by a separate Seegene PneumoBacter ACE detection panel, which also offers Ct values for the detected analytes.

Techniques: RP Assay

Journal: Diagnostic Microbiology and Infectious Disease

Article Title: FilmArray® respiratory panel performance in respiratory samples from neonatal care units

doi: 10.1016/j.diagmicrobio.2014.02.010

Figure Lengend Snippet: Combination of multiple viruses identified using FilmArray RP in archived samples from neonatal patients.

Article Snippet: Positive percentage agreement and negative percentage agreement were assessed for FilmArray RP assay with respect to the both laboratory-developed real-time RT-PCR and the Seegene Seeplex RV15® assay.

Techniques:

Journal: Diagnostic Microbiology and Infectious Disease

Article Title: FilmArray® respiratory panel performance in respiratory samples from neonatal care units

doi: 10.1016/j.diagmicrobio.2014.02.010

Figure Lengend Snippet: Overall concordant, discordant, and agreement results for laboratory-developed real-time RT-PCR panel and Seegene Seeplex RV15® assay compared with FilmArray.

Article Snippet: Positive percentage agreement and negative percentage agreement were assessed for FilmArray RP assay with respect to the both laboratory-developed real-time RT-PCR and the Seegene Seeplex RV15® assay.

Techniques: Quantitative RT-PCR, Reverse Transcription Polymerase Chain Reaction

Journal: Diagnostic Microbiology and Infectious Disease

Article Title: FilmArray® respiratory panel performance in respiratory samples from neonatal care units

doi: 10.1016/j.diagmicrobio.2014.02.010

Figure Lengend Snippet: Detection results for laboratory-developed real-time RT-PCR panel and Seegene Seeplex RV15® assay compared with FilmArray.

Article Snippet: Positive percentage agreement and negative percentage agreement were assessed for FilmArray RP assay with respect to the both laboratory-developed real-time RT-PCR and the Seegene Seeplex RV15® assay.

Techniques: Quantitative RT-PCR, Infection

Journal: Diagnostic Microbiology and Infectious Disease

Article Title: FilmArray® respiratory panel performance in respiratory samples from neonatal care units

doi: 10.1016/j.diagmicrobio.2014.02.010

Figure Lengend Snippet: Differences between laboratory-developed real-time RT-PCR panel or Seegene Seeplex RV15® results and FilmArray results.

Article Snippet: Positive percentage agreement and negative percentage agreement were assessed for FilmArray RP assay with respect to the both laboratory-developed real-time RT-PCR and the Seegene Seeplex RV15® assay.

Techniques: Quantitative RT-PCR, RP Assay

Journal: Clinics in Laboratory Medicine

Article Title: Molecular Detection of Respiratory Viruses

doi: 10.1016/j.cll.2013.03.007

Figure Lengend Snippet: BioFire FilmArray respiratory panel assay. ( A ) FilmArray RP Pouch. ( B ) FilmArray instrument and pouch. Instrument footprint in inches: 10.00 width × 6.5 height × 15.5 depth. A computer is required for operation.

Article Snippet: BioFire FilmArray respiratory panel assay. ( A ) FilmArray RP Pouch. ( B ) FilmArray instrument and pouch.

Techniques: